Advanced Instrumentation Techniques Free Notes Download – B. Pharm

Unit I

Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications
Mass Spectrometry- Principles, Fragmentation, Ionization techniques – Electron impact, chemical ionization, MALDI, FAB, Analyzers-Time of flight and Quadrupole, instrumentation, applications

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Unit II

Thermal Methods of Analysis: Principles, instrumentation and applications of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X- Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X- ray
Crystallography, rotating crystal technique, single crystal diffraction,powder diffraction, structural elucidation and applications.

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Unit III

Calibration and validation-as per ICH and USFDA guidelines
Calibration of following Instruments

Electronic balance, UV-Visible spectrophotometer, IR spectrophotometer,Fluorimeter, Flame Photometer, HPLC and GC

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Unit IV

Radio immune assay:

Importance, various components, Principle, different methods, Limitation and Applications of Radio immuno assay
Extraction techniques:General principle and procedure involved in the solid phase extraction and liquid-liquid extraction

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Unit V

Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS.

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